Protocols

Equipment and Software

Spin-caster Protocol
Centrifuge Protocol
Nikon Camera Control
Freeze-Dryer Protocol

MIMS Procedures

MIMS Procedures

Quantitative Image Analysis Procedures
Sample Preparation
Fixing, Staining, Embedding, Trimming, and Microtomy
Sample Holders and Silicon Chips
*Please note: These protocols are works in progress. Users are strongly encouraged to contact the NRIMS staff prior to using one of these protocols to verify that it is current.

We encourage you to contact us with any questions, feedback, comments, or corrections.

spin-caster_protocol.pdf852 KB
use_of_centrifuge.pdf47 KB
nikon_camera.pdf62 KB
freeze_dryer_protocol.pdf70 KB
mims_procedures_080910.pdf4.16 MB
image-procedures.pdf4.33 MB
roi_method.pdf1.11 MB
embedding_media.pdf14 KB
routine_embedding_method.pdf81 KB
fgp_fix.pdf21 KB
osmium_tetroxide_fix.pdf13 KB
toluidine_blue_stain.pdf13 KB
uranyl_acetate_stain_in_maleate_buffer.pdf15 KB
washing_and_sterilizing_of_silicon_pieces.pdf93 KB
washing_sample_holders.pdf14 KB